Tag Archives: Interferon

this press for “unlikely” situations: Ebolavirus VP35 is a multifunctional virulence factor


English: Biosafety level 4 hazmat suit: resear...

English: Biosafety level 4 hazmat suit: researcher is working with the Ebola virus (Photo credit: Wikipedia)

Nurse-nun visits graves of victims of 1976 Zai...

Nurse-nun visits graves of victims of 1976 Zaire Ebola outbreak (Photo credit: Wikipedia)

English: Ebola virus virion. Created by CDC mi...

English: Ebola virus virion. Created by CDC microbiologist Cynthia Goldsmith, this colorized transmission electron micrograph (TEM) revealed some of the ultrastructural morphology displayed by an Ebola virus virion. (Photo credit: Wikipedia)

English: Color-enhanced electron micrograph of...

English: Color-enhanced electron micrograph of Ebola virus particles. Polski: Mikrofotografia elektronowa cząsteczek wirusa Ebola w fałszywych kolorach. (Photo credit: Wikipedia)

The isolation ward of Gulu Municipal Hospital,...

The isolation ward of Gulu Municipal Hospital, Gulu, Uganda, during an outbreak of Ebola hemorrhagic fever in October 2000 (Photo credit: Wikipedia)

Abstract

Ebola virus (EBOV) is a member of the filoviridae family that causes severe hemorrhagic fever during sporadic outbreaks, and no approved treatments are currently available. The multifunctional EBOV VP35 protein facilitates immune evasion by antagonizing antiviral signaling pathways and is important for viral RNA synthesis. In order to elucidate regulatory mechanisms and to develop countermeasures, we recently solved the structures of the Zaire and Reston EBOV VP35 interferon inhibitory domain (IID) in the free form and of the Zaire EBOV VP35 IID bound to dsRNA. Together with biochemical, cell biological and virological studies, our structural work revealed that distinct regions within EBOV VP35 IID contribute to virulence through host immune evasion and viral RNA synthesis. Here we summarize our recent structural and functional studies and discuss the potential of multifunctional Ebola VP35 as a therapeutic target.

Key words: filoviruses, ebola virus, marburg virus, VP35, IFN antagonist, RNA binding protein, virulence factor, immune evasion, drug target

via Ebolavirus VP35 is a multifunctional virulence factor.